in vitro evaluation of capsaicin inhibitory effects on zonula occludens toxin in vibrio cholerae atcc14035 strain

نویسندگان

soroor erfanimanesh department of microbiology, shahid beheshti university of medical sciences, tehran, iran.

gita eslami department of microbiology, shahid beheshti university of medical sciences, tehran, iran.

hossein goudarzi department of microbiology, shahid beheshti university of medical sciences, tehran, iran.

arezou taherpour microbiology department, kurdistan university of medical sciences, sanandaj, iran

چکیده

background: cholera is a potentially epidemic and life-threatening secretory diarrhoea characterized by voluminous watery stools, often accompanied by vomiting, and resulting in hypovolemic shock and acidosis. it is caused by certain strains of the species vibrio cholerae which can also cause mild or in apparent infections. the aim of this study is the evaluation of capsaicin, as a potential inhibitor of zonula occludens toxin production in v. cholerae atcc 14035. materials and methods: mic of capsaicin was determined by broth microdilution method according to clsi guidelines. the zot gene expression level were analysed using real-time rt–pcr technique. results: results from mic test showed that 100 μg ml−1of capsaicin was the highest concentration that did not affect the bacterial growth however, zonula occludens toxin (zot) gene expression of the tested strain was significantly inhibited by capsaicin in a dose-dependent manner at sub-bacteriocidal concentrations. the reca gene did not show any significant difference in its expression with or without capsaicin. conclusion: capsaicin is one of the active compounds of red chili that can drastically suppress zot gene expression and shows promising inhibitory effect against v. cholerae zot production. thus, routine intake of red chilli, which is easily available and inexpensive, may be an alternative approach to prevent and control symptoms of cholera.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

In vitro evaluation of capsaicin inhibitory effects on zonula occludens toxin in vibrio cholerae ATCC14035 strain

Background: Cholera is a potentially epidemic and life-threatening secretory diarrhoea characterized by voluminous watery stools, often accompanied by vomiting, and resulting in hypovolemic shock and acidosis. It is caused by certain strains of the species Vibrio cholerae which can also cause mild or in apparent infections. The aim of this study is the evaluation of Capsaicin, as a potential in...

متن کامل

Capsaicin inhibitory effects on Vibrio cholerae toxin genes expression

Objective: Cholera is an acute secretory diarrhea caused by the Gram-negative bacterium, Vibrio cholerae mostly through production of cholera toxin (CT) and zonula occludens toxin (Zot).<span style="fon...

متن کامل

Molecular Detection of Zonula Occludens Toxin (zot) Genes in Vibrio Cholerae O1 using PCR

Background: Zot (Zonula occludens toxin) is one of the secretion toxins of Vibrio cholerae in small intestine that binds to certain receptors in the epithelial cells and causes a change in the structure of tight junction. The purpose of this research is rapid detection of zot enterotoxin gene using PCR. Materials and Methods: The genomic DNA was extracted by DNA isolation kit and gene amplific...

متن کامل

Gene encoding zonula occludens toxin (zot) does not occur independently from cholera enterotoxin genes (ctx) in Vibrio cholerae.

Of 167 Vibrio cholerae isolates screened for sequences homologous with zonula occludens toxin (zot) or cholera toxin (ctx) genes, 3.0% of non-O1, 100.0% of clinical O1, and 0.0% of environmental O1 strains contained both zot and ctx. zot was present only in strains that were ctx positive; all ctx-positive strains carried zot. The absence of zot-positive, ctx-negative strains suggests ZOT is not...

متن کامل

Distribution of the z o t gene among strains of and non - 01 ( zonula occludens Vibrio cholerae 01 toxin )

The distribution of the zot gene that encodes the zonula occludens toxin, a newly described toxin of Pibrio cholerae, among clinical, environmental and food isolates of V. cholerae 01 and non-01 was investigated. Both the zot gene and the ctx gene that encode cholera toxin were found in 247 of 257 clinical strains and 62 of 415 environmental or food isolates of V. cholerae 01. The zot gene, but...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
research in molecular medicine

جلد ۲، شماره ۴، صفحات ۳۳-۳۵

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023